NUCLEIC_ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
ELECTRO-
PHORESIS
CUSTOM
SERVICES
Isolation of Total RNA Isolation of DNA Isolation of Total
RNA/DNA/Proteins
RNA/DNA Isolation Reagents

RNA/DNA Isolation Reagents

-Agarose
From Pseudomonas atlantica

Key facts:

  • purified from Pseudomonas atlantica
  • allows high yield and rapid recovery of nucleic acids from GenAgarose LM
  • delivered in ready-to-use solution

Specifications:

Concentration : 100 units/l; solubilized in 10mM sodium phosphate buffer (pH 6.1) stabilized with 0.01% BSA, 50% glycerol; 10X reaction buffer is supplied with the enzyme.

-agarose is used for the recovery of pure DNA and RNA from agarose low melting gels (Ishimatsu K. et al. (1956) Sci. and Ind. (Japan), 30, 137) . One b-agarase unit will solubilize 500 mg of low melting agarose gel at 40-42C within one hour. To isolate nucleic acids from the agarose gel, cut out the band from the gel as accurately as possible; add to the slice half of the volume (or weight) of 1X reaction buffer and melt the agarose at 40-42C. Add one unit b-agarase per 500l melted agarose and allow to incubate at 40-42 for one hour. Nucleic acids can then be recovered by a simple ethanol precipitation. Storage and Shipping:

-agarose should be stored at -20C; shipped refrigerated.

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DEPC
Diethylpyrocarbonate
Specifications:

Assay : min. 97%; refractive index (n 20/D) : 1.398; density (d 20/4) : 1.122 g/ml; boiling point : 160-163C.

Storage:

store at 2-8C.

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Guanidine Hydrochloride
Guanidinium chloride molecular biology grade
Specifications:

Assay : min. 99.5%; melting point : 184-186C; pH (6M in water) : 4.5-6.0 solubility (20C) : 2000g/l; A (1cm/50% in water ) 230 nm : max. 0.1; A (1cm/50% in water ) 260 nm : max. 0.01; chloride : max. 0.001%; cyanide : max. 0.0005%; nitrate : max. 0.005%; sulfate : max. 0.005%; Pb : max. 0.00005%; Fe : max. 0.0002%; Cu : max. 0.00002%; water : max. 0.5%.

Storage:

store at room temperature.

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Guanidine Isothiocyanate
Guanidine Thiocyanate, Guanidine Rhodanide molecular biology grade
Specifications:

Assay : min. 99.0%; melting point : 116-118C; A (1cm/6M in water ) 280 nm : max. 0.3; A (1cm/6M in water ) 300 nm : max. 0.05; Fe : max. 0.0001%; water : max. 0.3%.

Storage:

store at room temperature.

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Lithium Chloride
Analytical grade
Specifications:

Assay : min. 99.0%; solubility (water, 20C) : 820 g/l; melting point : 614C; sulfate : max. 0.01%; As : max. 0.005%; Ca : max. 0.01%; Fe : max. 0.005%; Na : max. 0.01%.

Storage:

store at room temperature.

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Phenol
Carbolic acid molecular biology grade
Specifications:

Assay : min. 99.8%; solubility (water, 20C) : 90 g/l; pH (0.5M in water, 25C) : 4.0-5.0; melting point : 40-41.5C; boiling point : 180-182C; water : max. 0.05%.

Storage:

store at room temperature, in a dry place.

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RNase-EX
RNase decontamination solution
Key facts:
  • completely removes RNase contamination from glass or plastic surfaces
  • inactivates more than 20g RNase A
  • non-alkaline, non-corrosive, non-carcinogenic
  • heat-resistant
  • stable
  • ready-to-use

Specifications:

RNase-EX™ is ready-to-use for eliminating RNase from any surface including the interior of microcentrifuge tubes. By following the simple decontamination protocol given, RNase is completely inactivated and removed. When reaction vessels are cleaned all potentially enzyme-inhibitory residues from the cleaning solution are removed.

Storage and Shipping:

RNase-Ex should be stored at room

For further information and prizes about these products send us an eMail or use our online order forms.