|PCR & RELATED
|M-MuLV REVERSE TRANSKRIPTASE RNase H minus||Online Order|
M-MuLV REVERSE TRANSKRIPTASE - RNAse H minus MODIFIED
Murine Leukemia Virus (M-MuLV) Reverse Transkriptase is isolated from an E.Coli strain
that carries a plasmid with the cloned modified M-MuLV RT gene with deleted RNAse H coding
part. M-MuLV RTase is an RNA-dependent DNA polymerase. This enzyme can synthesize a
complementary DNA strand initiating from a primer using either single-stranded RNA or DNA
template. The enzyme lacks RNAse H activity.
one unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid precipitable form after 10 minutes at 37oC using poly(rA) -p(dT)10-20 as template/primer.
tested for the ability to synthesize full length cDNA from 4 kb RNA.
50 mM Tris-HCl pH 8,3, 1 mM EDTA, 0,1 mM DTT, 0,1mM NaCl, 0,1% Triton X-100, 50% glycerol.
|STORAGE AND SHIPPING:
store at -20 oC,
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