NUCLEIC_ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
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Isolation of Total RNA Isolation of DNA Isolation of Total
RNA/DNA/Proteins
RNA/DNA Isolation Reagents

Liquid Phenol

stabilized
Cat# Packsize Description Product composition
12606
12608


100 ml
500 ml


Phenol water saturated
Phenol saturated with water; stabilized with 0.1% 8-Hydroxyquinoline; pH (20C) : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.
pH 4.2-5
12597
12599
100 ml
500 ml
Phenol Tris saturated
Phenol extracted with Tris-EDTA buffer; stabilized with 0.1% 8-Hydroxyquinoline; pH (20C) : 7.6-8.0; heavy metals : max. 0.0005%; water : approx. 9.0%.
pH 7.6-8.0
12627
12629
100 ml
500 ml
Phenol equilibrated,Chloroform/Isoamyl alcohol 25:24:1Liquid
Composition : 500 ml phenol equilibrated with Tris, stabilized with 0.1% 8-Hydroxyquinoline - 480 ml chloroform - 20 ml isoamyl alcohol; pH (20C) : 7.6-8.0; heavy metals : max. 0.0005%; chloride : max. 0.0005%; water : approx. 9.0%.
pH 7.6-8.0
non stabilized
Cat# Packsize Description Product composition
12600
12602
100 ml
500 ml
Phenol water saturated
Phenol saturated with water; without 8-Hydroxyquinoline; pH (20C) : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.
pH 4.0
12608
12605
100 ml
500 ml
Phenol water saturated,  separate Tris buffer for pH adjustment
Phenol saturated with water without 8-Hydroxyquinoline delivered with a separate Tris buffer (50 ml,250 ml for 100 ml, 500 ml phenol solution respectively); by adding different quantities of the Tris buffer, corresponding pH values (pH 6.5 - 8.0) can be adjusted before use (see example on the table); pH without adjustment : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.
Phenol           starting volume                                       Tris buffer added  Final pH  value obtained  
100 ml   5 ml 6.500.1
100 ml 10 ml 6.880.1
100 ml 15 ml 7.150.1
100 ml 20 ml 7.350.1
100 ml 25 ml  7.480.1
12615
12617
100 ml
500 ml
Phenol water saturated,Chloroform/Isoamyl alcohol 25:24:1 + separate Tris buffer for pH adjustment Phenol           starting volume                Tris buffer added Final pH value obtained
100 ml   5 ml 7.380.1
100 ml 10 ml 7.590.1
100 ml 15 ml 7.760.1
100 ml 20 m 57.870.1
100 ml 25 ml   7.960.1

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