NUCLEIC_ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
ELECTRO-
PHORESIS
CUSTOM
SERVICES
Isolation of Total RNA Isolation of DNA Isolation of Total
RNA/DNA/Proteins
RNA/DNA Isolation Reagents

RNA/DNA Isolation Reagents

-Agarase
From Pseudomonas atlantica

Key facts :

  • purified from Pseudomonas atlantica
  • allows high yield and rapid recovery of nucleic acids from GenAgarose LM
  • delivered in ready-to-use solution
-agarase is used for the recovery of pure DNA and RNA from agarose low melting gels (Ishimatsu K. et al. (1956) Sci. and Ind. (Japan), 30, 137) . One b-agarase unit will solubilize 500 mg of low melting agarose gel at 40-42C within one hour. To isolate nucleic acids from the agarose gel, cut out the band from the gel as accurately as possible; add to the slice half of the volume (or weight) of 1X reaction buffer and melt the agarose at 40-42C. Add one unit b-agarase per 500l melted agarose and allow to incubate at 40-42 for one hour. Nucleic acids can then be recovered by a simple ethanol precipitation. Storage and Shipping:

-agarase should be stored at -20C; shipped refrigerated.

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DEPC
Diethylpyrocarbonate
Storage:

store at 2-8C.

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Guanidine Hydrochloride
Guanidinium chloride molecular biology grade
Storage:

store at room temperature.

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Guanidine Isothiocyanate
Guanidine Thiocyanate, Guanidine Rhodanide molecular biology grade
Storage:

store at room temperature.

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Lithium Chloride
Analytical grade
Storage:

store at room temperature.

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Phenol
Carbolic acid molecular biology grade
Storage:

store at room temperature, in a dry place.

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Phenol water saturated, stabilized
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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Phenol water saturated, non stabilized
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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Phenol tris saturated (pH 7.6-8.0), stabilized
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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Phenol water saturated, non stabilized + separate tris buffer for pH adjustment
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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Phenol equilibrated (pH 7.6-8.0), stabilized/chloroform/isoamyl, alcohol 25:24:1
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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Phenol water saturated, non stabilized/chloroform/isoamyl, alcohol 25:24:1 + separate tris buffer for pH adjustment
Liquid molecular biology grade
Storage:

store at 2-8C away from light (bottled under argon).

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RNase-EX
RNase decontamination solution
Storage and Shipping:

RNase-Ex should be stored at room

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