CUSTOM
SERVICES
NUCLEIC ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
ELECTRO-
PHORESIS
Custom Oligonucleotide Synthesis Order FISH-Service Custom DNA Sequencing Order Custom
Peptide
Synthesis Order

Custom Services for oligonucleotide synthesis and DNA sequencing

CUSTOM OLIGONUCLEOTIDE SYNTHESIS
GENAXIS Biotechnology offers high-quality, fast, custom synthesis and modification of oligonucleotides. Both our industrial production facility and expertise allow the synthesis of 5bp to more than 140bp in length without additional cost. GENAXIS Biotechnology is also able to synthesize crude or modified oligonucleotides in scales comprised between 10 nmoles to 10moles.

Quality: The quality and sequence of every oligonucleotide is guaranteed. Each oligonucleotide is deprotected, desalted and lyophilized so that it can be readily dissolved in water or buffer. Upon request we may deliver your oligonucleotides already solubilized in purified water or TE buffer. With each oligonucleotide is also delivered a certificate stating : - sequence synthetized, - name of the sequence, - GC ratio, - molar extinction coefficient, - quantity delivered in OD, nmoles and g - l water or buffer necessary to obtain a 50M solution - Tm value

Pricing: Prices for standard or cartridge purified oligonucleotides are set per base, with no set-up charges, mixed site charges or hidden fees. For modification, labeling or purification by PAGE or HPLC a set-up price is given.

To order: You will find a special order form here. When completed please return it by mail or contact your local distributor. If you need further technical assistance do not hesitate in contacting us or your nearest distributor.

CUSTOM DNA SEQUENCING
GENAXIS Biotechnology offers unique state of the art custom DNA sequencing services. Results obtained are of top quality owing to the expertise used in our production facility. All projects are treated confidentially.

ORDERING INFORMATION: To order our Sequencing services you will find a special order form.

Please pay attention to the pretended Primer- and Template-concentrations: Plasmid >3 g (200 ng/l) Vector (Clonig site / Insert size) Cosmid >9 g (600 ng/l) Standard-Primer (free of charge) PCR-fragment >1 g (50 ng/l) for ~1000 bp PCR-fragment >0,5 g (25 ng/l) for ~500 bp PCR-fragment >0,3 g (20 ng/l) for ~200 bp Primer (each) 5 l (10 pmol/l) 18 - 25 mer

Send your DNA only dissolved in Water (not in buffer !) and use the same name and marks on sample-tube and order-form.

FISH-AXIS: Fluorescence in situ Hybridization services
With GENAXIS Biotechnology, FISH is faster! We supply fast and reliable human chromosomal localization from your own DNA clones: genomic DNA or cDNA. These can be in the form of:
  • PCR product
  • BAC
  • PAC
  • YAC
  • Plasmid
  • Cosmid

For FISH-AXIS services please provide:

  • cDNA or DNA clones > 5 Kb (for DNA fragments between 1 and 5 Kb please contact us)
  • at least 5 - 10 g of DNA (solubilized in water)
  • the purification system you have used
  • the concentration of the DNA supplied
  • a picture of your DNA material after gel electrophoresis
Custom antibody production
GENAXIS Biotechnology offers the fast and high quality production of polyclonal antisera from your specific antigen. Antibody production is performed on selected animals. Mouse, rat, rabbit, guinea pig, cock-hen, sheep, goat are systematically available; horse, donkey and pig are available on request.

Our prices include animal housing, food, immunisation, taking of blood.

IMPORTANT: No extra cost for purchase of animal or its monthly maintenance! Freight costs are not included in our pricing.

Send your antigen:

  • lyophilized
  • in sloution in physiological buffer (e.g. PBS buffer pH 7.0-8.0)
  • included in a PAGE slice
  • immobilised on a nitro-cellulose membrane.

GENAXIS™ Biotechnology offers the fast and high quality production of polyclonal antisera from your specific antigen. Antibody production is performed on selected animals. Mouse, rat, rabbit, guinea pig, cock-hen, sheep, goat are systematically available; horse, donkey and pig are available on request. Our prices include animal housing, food, immunisation, taking of blood. IMPORTANT : No extra cost for purchase of animal or its monthly maintenance! Freight costs are not included in our pricing.

Send your antigen :-lyophilized-in solution in physiological buffer (e.g. PBS buffer pH 7.0-8.0)-included in a PAGE slice-immobilised on a nitro-cellulose membrane.

Custom peptide antibody production
In this integral peptide antibody production GENAXIS Biotechnology offers: synthesis of your peptide sequence (15 to 30 amino acids), specific conjugation to carrier proteins (Bovine Serum Albumin, Ovalbumin or Keyhole Limpet Hemacyanin), standard immunisation using a three month program for two rabbits.

ALSO AVAILABLE:

  • Peptide synthesis including peptide modifications, peptide conjugation to carrier proteins.
  • Antibody labelling: HRPeroxydase, Biotin, Fluorescence.
  • Antibody purification: affinity purification using A or G Sapharose, affinity purification of sera using the antigen matrix.
  • Antibody fragmentation: fab-fragments SCS-PAGE or ELISA analysed.

Please do not hesitate contacting us for further technical assistance or prices.