ELECTRO-
PHORESIS

NUCLEIC ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
CUSTOM
SERVICES
Acrylamide matrices Equipment Online Order

Acrylamide Matrices

Polyacrylamide gels are formed by the polymerisation of acrylamide and the cross-linking with a bifunctional agent, usually bisacrylamide (N,N-Methylenebisacrylamide). The porosity of the gel is determined by the concentration of acrylamide and the ratio acrylamide:cross-linker. GENAXIS™ Biotechnology currently offers three different grades of acrylamide and bisacrylamide compounds : 2X, 4X and Fluorescence grades. The Acrylamide and Bisacrylamide 2X are recrystallized twice i.e. a standard grade suitable for most electrophoresis requirements. Acrylamide and Bisacrylamide 4X are recrystallized four times, and are high purity matrices with very low conductivity, suitable for all demanding electrophoresis protocols. Lately, Acrylamide and Bisacrylamide Fluorescence grade are special matrices used in fluorescence methods such as automated sequencing of DNA.As acrylamide is a potent neurotoxic it is recommended to use ready-to-use acrylamide and bisacrylamide solutions. Also premixed solutions are available from GENAXIS™ Biotechnology in the current acrylamide/bisacrylamide ratios and solutions for the preparation of DNA denaturating sequencing gels (SEQUAMIX). Prepared with reagents of the highest purity in large batches these premixed solutions ensure high product reliability.

Powders

Ready-to-use solutions

Premixed solutions

When using our 40% stock solutions for SDS-PAGE (for proteins), non denaturating or denaturing PAGE (for DNA) gels, the stock solution should be diluted as follows :Total volume of gel solution x required concentration of monomer (%) = volume of stock solution % of stock solutionE. g. : for 100 ml of a 6% gel solution, made from a 40% acrylamide stock solution 29 : 1, mix 15 ml of the 40% stock solution with 10 ml of TBE buffer 10X and 70 ml water, calculating as follows :100 ml x 6% = 15 ml 40% stock solution40%

Acrymalide 4x

40% Acrylamide 4X, aqueous solution (w/v)

 

 

 

 

Premixed solutions are available in 3 acrylamide/bisacrylamide rations, 3 different qualities and as 40% stock solutions. These ready-to-use solutions are stable for 1 year at + 4C.

 

Acrymalide 2x

40% Acrylamide 2X, aqueous solution (w/v)

Sequamix 6 and Sequamix 8 solutions are premixed 6% and 8% Polyacrylamide/urea-solutions for denaturing sequencing gels.The user needs only to add amonium persulfate, mix and poor into a mold

Bisacrylamide Fluorescence grade

2% Bisacrylamide 4X, aqueous solution (w/v)

Bisacrylamide 4X

2% Bisacrylamide 2X, aqueous solution (w/v)

Bisacrylamide 2X

40% Acrylamide Fluorescence grade - Mix 19 : 1, aqueous solution (w/v)

40% Acrylamide 4X - Mix 19 : 1, aqueous solution (w/v)

40% Acrylamide 2X - Mix 19 : 1, aqueous solution (w/v)

40% Acrylamide Fluorescence grade - Mix 29 : 1, aqueous solution (w/v)

40% Acrylamide 4X - Mix 29 : 1, aqueous solution (w/v)

40% Acrylamide 2X - Mix 29 : 1, aqueous solution (w/v)

40% Acrylamide Fluorescence grade - Mix 37.5 : 1, aqueous solution (w/v)

40% Acrylamide 4X - Mix 37.5 : 1, aqueous solution (w/v)

40% Acrylamide 2X - Mix 37.5 : 1, aqueous solution (w/v)

Acrylamide 4X - Sequamix 6 solution

Acrylamide 4X - Sequamix 6 solution

For futher information and prizes about the following products send us an eMail or use our online order form.