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Acrylamide matrices  Equipment  Online Order 
Acrylamide Matrices
Polyacrylamide gels are formed by the polymerisation of acrylamide and the crosslinking with a bifunctional agent, usually bisacrylamide (N,NMethylenebisacrylamide). The porosity of the gel is determined by the concentration of acrylamide and the ratio acrylamide:crosslinker. GENAXIS™ Biotechnology currently offers three different grades of acrylamide and bisacrylamide compounds : 2X, 4X and Fluorescence grades. The Acrylamide and Bisacrylamide 2X are recrystallized twice i.e. a standard grade suitable for most electrophoresis requirements. Acrylamide and Bisacrylamide 4X are recrystallized four times, and are high purity matrices with very low conductivity, suitable for all demanding electrophoresis protocols. Lately, Acrylamide and Bisacrylamide Fluorescence grade are special matrices used in fluorescence methods such as automated sequencing of DNA.As acrylamide is a potent neurotoxic it is recommended to use readytouse acrylamide and bisacrylamide solutions. Also premixed solutions are available from GENAXIS™ Biotechnology in the current acrylamide/bisacrylamide ratios and solutions for the preparation of DNA denaturating sequencing gels (SEQUAMIX). Prepared with reagents of the highest purity in large batches these premixed solutions ensure high product reliability. 
Powders 
Readytouse solutions 

When using our 40% stock solutions for SDSPAGE (for proteins), non denaturating or denaturing PAGE (for DNA) gels, the stock solution should be diluted as follows :Total volume of gel solution x required concentration of monomer (%) = volume of stock solution % of stock solutionE. g. : for 100 ml of a 6% gel solution, made from a 40% acrylamide stock solution 29 : 1, mix 15 ml of the 40% stock solution with 10 ml of TBE buffer 10X and 70 ml water, calculating as follows :100 ml x 6% = 15 ml 40% stock solution40%  
Acrymalide 4x 
40% Acrylamide 4X, aqueous solution (w/v)

Premixed solutions are available in 3 acrylamide/bisacrylamide rations, 3 different qualities and as 40% stock solutions. These readytouse solutions are stable for 1 year at + 4°C.

Acrymalide 2x 
40% Acrylamide 2X, aqueous solution (w/v) 
Sequamix 6 and Sequamix 8 solutions are premixed 6% and 8% Polyacrylamide/ureasolutions for denaturing sequencing gels.The user needs only to add amonium persulfate, mix and poor into a mold 
Bisacrylamide Fluorescence grade 
2% Bisacrylamide 4X, aqueous solution (w/v)  
Bisacrylamide 4X 
2% Bisacrylamide 2X, aqueous solution (w/v)  
Bisacrylamide 2X 
40% Acrylamide Fluorescence grade  Mix 19 : 1, aqueous solution (w/v)  
40% Acrylamide 4X  Mix 19 : 1, aqueous solution (w/v)  
40% Acrylamide 2X  Mix 19 : 1, aqueous solution (w/v)  
40% Acrylamide Fluorescence grade  Mix 29 : 1, aqueous solution (w/v)  
40% Acrylamide 4X  Mix 29 : 1, aqueous solution (w/v)  
40% Acrylamide 2X  Mix 29 : 1, aqueous solution (w/v)  
40% Acrylamide Fluorescence grade  Mix 37.5 : 1, aqueous solution (w/v)  
40% Acrylamide 4X  Mix 37.5 : 1, aqueous solution (w/v)  
40% Acrylamide 2X  Mix 37.5 : 1, aqueous solution (w/v)  
Acrylamide 4X  Sequamix 6 solution  
Acrylamide 4X  Sequamix 6 solution 
For futher information and prizes about the following products send us an eMail or use our online order form.